Addgene - CaV2.1 (pcDNA6) Plasmid Data

Plasmid 26578: CaV2.1 (pcDNA6)

Gene/insert name:
Cacna1a
Alt name:
calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Insert size:
7100
Species:
M. musculus (mouse)
Entrez Gene:
Cacna1a (APCA, Caca1a, Cacnl1a4, Cav2.1, Ccha1a, EA2, FHM, HPCA, MHP, MHP1, SCA6, alpha1A, la, leaner, nmf352, rkr, rocker, tg, tottering)
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Fusion protein or tag:
His6
Terminal:
C terminal on backbone
Vector backbone:
pcDNA6/V5-HisA
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5100
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Top10F'
Growth temperature (℃):
37
Growth instructions:
Top10F'
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Diane Lipscombe
Terms and Licenses:
MTA

Comments:

The fragment was amplified from mouse brain. The GenBank sequence is AY714490.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Novel CaV2.1 clone replicates many properties of Purkinje cell CaV2.1 current. Richards et al (Eur J Neurosci. 2007 Nov . 26(10):2950-61. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26578" in your Materials and Methods section.