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CaV2.1 (pcDNA6)
(Plasmid #26578)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA6/V5-HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Growth instructions
    Top10F'
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Cacna1a
  • Alt name
    calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7100
  • Entrez Gene
    Cacna1a (a.k.a. APCA, BI, Caca1a, Cacnl1a4, Cav2.1, Ccha1a, EA2, FHM, HPCA, MHP, MHP1, SCA6, alpha1A, la, nmf352, rkr, tg)
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • His6 (C terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The fragment was amplified from mouse brain. The GenBank sequence is AY714490.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaV2.1 (pcDNA6) was a gift from Diane Lipscombe (Addgene plasmid # 26578)
  • For your References section:

    Novel CaV2.1 clone replicates many properties of Purkinje cell CaV2.1 current. Richards KS, Swensen AM, Lipscombe D, Bommert K. Eur J Neurosci. 2007 Nov . 26(10):2950-61. 10.1111/j.1460-9568.2007.05912.x PubMed 18001290