Addgene - pSIH1-puro-STAT3 shRNA Plasmid Data

Plasmid 26596: pSIH1-puro-STAT3 shRNA

Gene/insert name:
STAT3 shRNA
Insert size:
60
Species:
H. sapiens (human)
Entrez Gene:
STAT3 (APRF, HIES)
Vector backbone:
pSIH1-H1-puro
(Search Vector Database)
Backbone manufacturer:
System Biosciences
Vector type:
Lentiviral, RNAi
Backbone size w/o insert (bp):
7200
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
agtgtcactaggcgggaacac List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Frank Sinicrope
Terms and Licenses:
MTA

Comments:

STAT3 shRNA sequence: gatccGCATCTGCCTAGATCGGCTATTCAAGAGATAGCCGATCTAGGCAGATGTTTTTTg

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Sorafenib inhibits STAT3 activation to enhance TRAIL-mediated apoptosis in human pancreatic cancer cells. Huang et al (Mol Cancer Ther. 2010 Mar . 9(3):742-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26596" in your Materials and Methods section.