Addgene - nanos-PCRII Plasmid Data

Plasmid 26601: nanos-PCRII

Gene/insert name:
nanos
Insert size:
918
Species:
S. mediterranea (planarian)
GenBank ID:
ef035555
Vector backbone:
PCRII
(Search Vector Database)
Backbone size w/o insert (bp):
4000
Cloning method:
TOPO Cloning
Cloning site 5':
T/A Cloning
Site destroyed during cloning:
Yes
Cloning site 3':
T/A Cloning
Site destroyed during cloning:
Yes
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
M13pUC-fwd
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Phillip Newmark
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: nanos function is essential for development and regeneration of planarian germ cells. Wang et al (Proc Natl Acad Sci U S A. 2007 Apr 3. 104(14):5901-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26601" in your Materials and Methods section.