Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pNF579
(Plasmid #26616)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26616 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKSV7

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    HB101
  • Growth instructions
    In HB101 cells. Use 50ug/ml Amp
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gfpuv
  • Tag / Fusion Protein
    • gram-positive ribosome binding site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer GFP-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    gfpuv coding sequence was amplified from plasmid pGFPuv (Clontech).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gram-positive ribosome binding site (AGGAGGA) derived from SD1 of ermC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNF579 was a gift from Nancy Freitag (Addgene plasmid # 26616 ; http://n2t.net/addgene:26616 ; RRID:Addgene_26616)
  • For your References section:

    Listeria monocytogenes-infected phagocytes can initiate central nervous system infection in mice. Drevets DA, Jelinek TA, Freitag NE. Infect Immun. 2001 Mar . 69(3):1344-50. 10.1128/IAI.69.3.1344-1350.2001 PubMed 11179297