pET21a AHU
(Plasmid
#26640)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET21a
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAHU
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SpeciesAnabaena
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Insert Size (bp)295
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses with RJ1878 ( Kan. resistant ) which is HU-free expression strain from BL21(DE3) family, from Reid Johnson.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a AHU was a gift from Phoebe Rice (Addgene plasmid # 26640 ; http://n2t.net/addgene:26640 ; RRID:Addgene_26640) -
For your References section:
Flexible DNA bending in HU-DNA cocrystal structures. Swinger KK, Lemberg KM, Zhang Y, Rice PA. EMBO J. 2003 Jul 15. 22(14):3749-60. 10.1093/emboj/cdg351 PubMed 12853489
