|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26699||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5600
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name3x MHC I KB element - luciferase
Alt nameMHC I NFKB element
SpeciesH. sapiens (human); Photinus pyralis
Insert Size (bp)200
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer EBV Reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBaldwin, A. S., Jr., J. C. Azizkhan, D. E. Jensen, A. A. Beg, and L. R. Coodly. 1991. Induction of NF-KB DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts. Mol. Cell. Biol. 11:4943–4951.
Terms and Licenses
Articles Citing this Plasmid
The plasmid 3X-KB-L has a minimal fos promoter element and three copies of the major histocompatibility complex (MHC) class I KB element TGGGGATTCCCCA. It was constructed by ligation of the fragment obtained through digestion of MHC-NF-KB CAT by HindIII, blunting with Klenow DNA polymerase, and then digestion with BamHI. The fragment was ligated to pGL2-Basic digest(SmaI+BglII).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p1242 3x-KB-L was a gift from Bill Sugden (Addgene plasmid # 26699 ; http://n2t.net/addgene:26699 ; RRID:Addgene_26699)
For your References section:Stimulation of NF-kappa B-mediated transcription by mutant derivatives of the latent membrane protein of Epstein-Barr virus. Mitchell T, Sugden B. J Virol. 1995 May . 69(5):2968-76. PubMed 7707523