Addgene - pLKO.1-puro-cfPar3

Plasmid 26706: pLKO.1-puro-cfPar3_4

Gene/insert name:
Dog Par3 RNAi
Species:
C. familiaris (dog, RNAi)
Mutation:
Dog Par3 RNAi
Vector backbone:
pLKO.1-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7000
Cloning site 5':
AgeI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
Yes
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
RNAi sequences adapted for pLKO.1 from PMID: 18070914.
Sequence: View sequences (1)
Principal Investigator:
Keith Mostov
Terms and Licenses:
MTA

Comments:

Dog RNAi to Par3: 5'-GGCTTCGGGTGAATGATCAACTGATA-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A molecular network for de novo generation of the apical surface and lumen. Bryant et al (Nat Cell Biol. 2010 Oct 3. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26706" in your Materials and Methods section.