- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namedog Rab25 RNAi
SpeciesC. familiaris (dog, RNAi)
MutationRNAi to canine Rab25.
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Terms and Licenses
Matches dog and Panda Rab25. RNAi sequence is: 5'-AGAGATCTTCACCAAAGTG-3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1-blast-cfRab25_2 was a gift from Keith Mostov (Addgene plasmid # 26711)
For your References section:A molecular network for de novo generation of the apical surface and lumen. Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, Martin-Belmonte F, Mostov KE. Nat Cell Biol. 2010 Oct 3. ():. 10.1038/ncb2106 PubMed 20890297
Map generated by Addgene from full sequence supplied by depositor.