Addgene - pDREV-2 Plasmid Data

Plasmid 26752: pDREV-2

Gene/insert name:
H2B-Venus CDS
Alt name:
HIST1H2BB, H2B.1, H2B/f, H2BFF, MGC119804
Insert size:
1101
Species:
H. sapiens (human)
GenBank ID:
NM_021058
Entrez Gene:
HIST1H2BB (H2B.1, H2B/f, H2BFF)
Fusion protein or tag:
Venus
Terminal:
C terminal on insert
Vector backbone:
pL1L2_gt2
(Search Vector Database)
Backbone manufacturer:
B. Rosen and W.C.Skarnes, unpublished
Vector type:
Mammalian Expression, Cre/Lox ; FLP/FRT; DRE/Rox
Backbone size w/o insert (bp):
4500
Cloning site 5':
BlgII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
FCHK2 sequence: GTATCTGCAACCTCAAGCTAGC List of Sequencing Primers
Bacterial resistance(s)
ClonNAT (nourseothricin)
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
PGK-Puro cassette from Addgene plasmid 11349
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Rolf Zeller
Terms and Licenses:
MTA

Comments:

The pDREV plasmid series encode a 5' FRT site and a 3' loxP site for re-engineering of the IKMC knock-out first alleles by dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521

Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocol...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Dual RMCE for efficient re-engineering of mouse mutant alleles Osterwalder et al (Nat Methods. 2010 Oct 17 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26752" in your Materials and Methods section.

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