pEGIP
(Plasmid #26777)

• Purpose: Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.
• Depositing Lab: Linzhao Cheng
• Publication: Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Backbone

• Vector backbone: Duet011
• Backbone size w/o insert (bp): 7063
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin ; GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EF1a-GFP-IRES-Puro
• Alt name: GFP
• Alt name: Puromycin resistant gene
• Alt name: EF1alpha promoter
• Insert Size (bp): 3229
• Entrez Gene: gfp (a.k.a. pCmGFP_001)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: HpaI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 34 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Allows for puromycin selection of stable integration of GFP expression cassette. With modification, this vector enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting.