Addgene - lag-2 promoter:GFP (pJK590) Plasmid Data

Plasmid 26814: lag-2 promoter:GFP (pJK590)

Gene/insert name:
lag-2 promoter
Insert size:
3000
Species:
C. elegans (nematode)
Fusion protein or tag:
GFP
Terminal:
C terminal on backbone
Vector backbone:
pPD95.81
(Search Vector Database)
Backbone manufacturer:
Andrew Fire (Addgene Plasmid #1497)
Vector type:
Worm Expression
Backbone size w/o insert (bp):
4492
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
M13_Rev List of Sequencing Primers
3' sequencing primer:
GFP-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Judith Kimble
Terms and Licenses:
MTA

Comments:

Quick confirmation: BamHI should result in two fragments: ~3kb and 4.5kb.

This is a lag-2 promoter::GFP fusion. Strong GFP in live worms. 3kb BamHI fragment of pJK520 is the complete 3kb 5' region of lag-2.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: APX-1 can substitute for its homolog LAG-2 to direct cell interactions throughout Caenorhabditis elegans development. Gao et al (Proc Natl Acad Sci U S A. 1995 Oct 10. 92(21):9839-42. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26814" in your Materials and Methods section.