Addgene - pcDNA3.3_KLF4 Plasmid Data

Plasmid 26815: pcDNA3.3_KLF4

Gene/insert name:
5'UTR-KLF4-3'UTR
Alt name:
KLF4
Insert size:
1596
Species:
H. sapiens (human)
Entrez Gene:
KLF4 (RP11-150J11.1, EZF, GKLF)
Mutation:
Starts from second ATG
Vector backbone:
pcDNA3.3
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
5' sequencing primer:
CMV Forward List of Sequencing Primers
3' sequencing primer:
TK polyA reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Supplemental document:
Change to mRNA reprogramming protocol (application/x-pdf)
Principal Investigator:
Derrick Rossi
Terms and Licenses:
MTA

Comments:

Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Warren et al (Cell Stem Cell. 2010 Sep 29. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26815" in your Materials and Methods section.