pcasper4 p [7-Gr66a-]
(Plasmid #26860)

• Depositing Lab: Craig Montell
• Publication: Moon et al Curr Biol. 2006 Sep 19. 16(18):1812-7.

Backbone

• Vector backbone: pCaSper4
• Backbone size w/o insert (bp): 7855
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gr66 gustatory receptor
• Alt name: Gr66a
• Species: D. melanogaster (fly)
• Insert Size (bp): 7000
• Mutation: Partial deletion of Gr66a (see associated paper listed below for details), but contains genes CG7066 and CG7188, which neighbor Gr66a
• Entrez Gene: Gr66a (a.k.a. CG7189, Dmel\CG7189, Gr66A, GR66A, Gr66C1, GR66C1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry