pFA6a-HTB-hphMX4
(Plasmid #26876)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pFA6ahphMX4
  • Backbone size w/o insert (bp) 4400
  • Vector type
    PCR tagging yeast
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    HTB tag
  • Insert Size (bp)
    358

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pac1 (not destroyed)
  • 3′ cloning site asc1 (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-HTB-hphMX4 was a gift from Peter Kaiser (Addgene plasmid # 26876)
  • For your References section:

    HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae. Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, Auer B, Huang L, Kaiser P. Yeast. 2006 Jun . 23(8):623-32. 10.1002/yea.1380 PubMed 16823883