Addgene - pFA6a-kanMX6-PGAL1-HBH Plasmid Data

Plasmid 26885: pFA6a-kanMX6-PGAL1-HBH

Gene/insert name:
HBH tag N-terminal
Insert size:
296
Vector backbone:
pFA6a-kanMX6-PGAL1
(Search Vector Database)
Vector type:
PCR tagging yeast
Backbone size w/o insert (bp):
4700
Cloning site 5':
Pac1
Site destroyed during cloning:
No
Cloning site 3':
Asc1
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Gentamicin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Peter Kaiser
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: HB tag modules for PCR-based gene tagging and tandem affinity purification in Saccharomyces cerevisiae. Tagwerker et al (Yeast. 2006 Jun . 23(8):623-32. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 26885" in your Materials and Methods section.