- Backbone size w/o insert (bp) 6758
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert nameChe9c genes 60-61
Insert Size (bp)1998
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer sequence
- 3′ sequencing primer sequence (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJV63 was a gift from Graham Hatfull (Addgene plasmid # 26906)
For your References section:Recombineering in Mycobacterium tuberculosis. van Kessel JC, Hatfull GF. Nat Methods. 2007 Feb . 4(2):147-52. 10.1038/nmeth996 PubMed 17179933
Map generated by Addgene from plasmid data.