Addgene - pRS-puro shNEDD4L #1 Plasmid Data

Plasmid 27016: pRS-puro shNEDD4L #1

Gene/insert name:
shRNA NEDD4L #1
Alt name:
NEDD4L
Species:
H. sapiens (human)
Vector backbone:
pRetroSuper
(Search Vector Database)
Vector type:
Mammalian Expression, RNAi
Backbone size w/o insert (bp):
6401
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
pLXSN-5 List of Sequencing Primers
3' sequencing primer:
MSCV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Joan Massague
Terms and Licenses:
MTA

Comments:

shRNA sequence - 5'-gatccccGCTAGACTGTGGATTGAGTttcaagagaACTCAATCCACAGTCTAGCtttttggaaa

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta signaling. Gao et al (Mol Cell. 2009 Nov 13. 36(3):457-68. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27016" in your Materials and Methods section.