Addgene - pOPTN-EGFP Plasmid Data

Plasmid 27052: pOPTN-EGFP

Gene/insert name:
optineurin
Alt name:
OPTN
Insert size:
1738
Species:
H. sapiens (human)
GenBank ID:
NM_001008211
Entrez Gene:
OPTN (RP11-730A19.1, ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clonetech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4733
Cloning site 5':
ECoRI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
sequence List of Sequencing Primers
3' sequencing primer:
sequence
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Dr. Robert Weil, Institute Pasteur, Paris, France
Sequence: View sequences (2)
Principal Investigator:
Beatrice Yue
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Studies of optineurin, a glaucoma gene: Golgi fragmentation and cell death from overexpression of wild-type and mutant optineurin in two ocular cell types. Park et al (Am J Pathol. 2006 Dec . 169(6):1976-89. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27052" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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