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pCXLE-EGFP Citations (53)

Originally described in: A more efficient method to generate integration-free human iPS cells.
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S Nat Methods. 2011 May;8(5):409-12
PubMed Journal

Articles Citing pCXLE-EGFP

Articles
To clone or not to clone? Induced pluripotent stem cells can be generated in bulk culture. Willmann CA, Hemeda H, Pieper LA, Lenz M, Qin J, Joussen S, Sontag S, Wanek P, Denecke B, Schuler HM, Zenke M, Wagner W. PLoS One. 2013 May 29;8(5):e65324. doi: 10.1371/journal.pone.0065324. Print 2013. PubMed
A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells. Goh PA, Caxaria S, Casper C, Rosales C, Warner TT, Coffey PJ, Nathwani AC. PLoS One. 2013 Nov 26;8(11):e81622. doi: 10.1371/journal.pone.0081622. eCollection 2013. PubMed
Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. McCracken KW, Cata EM, Crawford CM, Sinagoga KL, Schumacher M, Rockich BE, Tsai YH, Mayhew CN, Spence JR, Zavros Y, Wells JM. Nature. 2014 Dec 18;516(7531):400-4. doi: 10.1038/nature13863. Epub 2014 Oct 29. PubMed
Laser-assisted generation of human induced pluripotent stem cells. Liu Y, Wu D, Lao D, Peterson C, Hohenstein Elliott KA, Snyder EY. Curr Protoc Stem Cell Biol. 2014 Nov 3;31:4A.7.1-4A.7.15. doi: 10.1002/9780470151808.sc04a07s31. PubMed
Efficient iPS cell generation from blood using episomes and HDAC inhibitors. Hubbard JJ, Sullivan SK, Mills JA, Hayes BJ, Torok-Storb BJ, Ramakrishnan A. J Vis Exp. 2014 Oct 28;(92):e52009. doi: 10.3791/52009. PubMed
Dynamic transcriptional and epigenomic reprogramming from pediatric nasal epithelial cells to induced pluripotent stem cells. Ji H, Zhang X, Oh S, Mayhew CN, Ulm A, Somineni HK, Ericksen M, Wells JM, Khurana Hershey GK. J Allergy Clin Immunol. 2015 Jan;135(1):236-44. doi: 10.1016/j.jaci.2014.08.038. Epub 2014 Oct 14. PubMed
Modeling Axonal Phenotypes with Human Pluripotent Stem Cells. Denton KR, Xu CC, Li XJ. Methods Mol Biol. 2014 Dec 18. PubMed
Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions. Caxaria S, Arthold S, Nathwani AC, Goh PA. Methods Mol Biol. 2015 Feb 21. PubMed
Dual optical recordings for action potentials and calcium handling in induced pluripotent stem cell models of cardiac arrhythmias using genetically encoded fluorescent indicators. Song L, Awari DW, Han EY, Uche-Anya E, Park SH, Yabe YA, Chung WK, Yazawa M. Stem Cells Transl Med. 2015 May;4(5):468-75. doi: 10.5966/sctm.2014-0245. Epub 2015 Mar 13. PubMed
Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3-dioxygenase1. Romani R, Pirisinu I, Calvitti M, Pallotta MT, Gargaro M, Bistoni G, Vacca C, Di Michele A, Orabona C, Rosati J, Pirro M, Giovagnoli S, Matino D, Prontera P, Rosi G, Grohmann U, Talesa VN, Donti E, Puccetti P, Fallarino F. J Cell Mol Med. 2015 Jul;19(7):1593-605. doi: 10.1111/jcmm.12534. Epub 2015 Mar 17. PubMed
An alternative pluripotent state confers interspecies chimaeric competency. Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause MN, Nery JR, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim NY, Lajara J, Guillen P, Campistol JM, Esteban CR, Ross PJ, Saghatelian A, Ren B, Ecker JR, Izpisua Belmonte JC. Nature. 2015 May 21;521(7552):316-21. doi: 10.1038/nature14413. Epub 2015 May 6. PubMed
Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature. Thomas SM, Kagan C, Pavlovic BJ, Burnett J, Patterson K, Pritchard JK, Gilad Y. PLoS Genet. 2015 May 7;11(5):e1005216. doi: 10.1371/journal.pgen.1005216. eCollection 2015 May. PubMed
Reprogramming fibroblasts toward cardiomyocytes, neural stem cells and hepatocytes by cell activation and signaling-directed lineage conversion. Zhu S, Wang H, Ding S. Nat Protoc. 2015 Jul;10(7):959-73. doi: 10.1038/nprot.2015.059. Epub 2015 Jun 4. PubMed
A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. Gallego Romero I, Pavlovic BJ, Hernando-Herraez I, Zhou X, Ward MC, Banovich NE, Kagan CL, Burnett JE, Huang CH, Mitrano A, Chavarria CI, Friedrich Ben-Nun I, Li Y, Sabatini K, Leonardo TR, Parast M, Marques-Bonet T, Laurent LC, Loring JF, Gilad Y. Elife. 2015 Jun 23;4:e07103. doi: 10.7554/eLife.07103. PubMed
Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes. Hung SS, Pebay A, Wong RC. J Vis Exp. 2015 Aug 20;(102):e53174. doi: 10.3791/53174. PubMed
Practical Integration-Free Episomal Methods for Generating Human Induced Pluripotent Stem Cells. Kime C, Rand TA, Ivey KN, Srivastava D, Yamanaka S, Tomoda K. Curr Protoc Hum Genet. 2015 Oct 6;87:21.2.1-21.2.21. doi: 10.1002/0471142905.hg2102s87. PubMed
Human iPS Cell-Derived Neurons Uncover the Impact of Increased Ras Signaling in Costello Syndrome. Rooney GE, Goodwin AF, Depeille P, Sharir A, Schofield CM, Yeh E, Roose JP, Klein OD, Rauen KA, Weiss LA, Ullian EM. J Neurosci. 2016 Jan 6;36(1):142-52. doi: 10.1523/JNEUROSCI.1547-15.2016. PubMed
Overcoming Pluripotent Stem Cell Dependence on the Repair of Endogenous DNA Damage. Chlon TM, Ruiz-Torres S, Maag L, Mayhew CN, Wikenheiser-Brokamp KA, Davies SM, Mehta P, Myers KC, Wells JM, Wells SI. Stem Cell Reports. 2016 Jan 12;6(1):44-54. doi: 10.1016/j.stemcr.2015.12.001. PubMed
Transcriptional Reactivation of OTX2, RX1 and SIX3 during Reprogramming Contributes to the Generation of RPE Cells from Human iPSCs. Li P, Sun X, Ma Z, Liu Y, Jin Y, Ge R, Hao L, Ma Y, Han S, Sun H, Zhang M, Li R, Li T, Shen L. Int J Biol Sci. 2016 Feb 20;12(5):505-17. doi: 10.7150/ijbs.14212. eCollection 2016. PubMed
Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro. Lesueur LL, Mir LM, Andre FM. Mol Ther Nucleic Acids. 2016 Mar 8;5:e291. doi: 10.1038/mtna.2016.4. PubMed
Plasmid-Based Generation of Induced Neural Stem Cells from Adult Human Fibroblasts. Capetian P, Azmitia L, Pauly MG, Krajka V, Stengel F, Bernhardi EM, Klett M, Meier B, Seibler P, Stanslowsky N, Moser A, Knopp A, Gillessen-Kaesbach G, Nikkhah G, Wegner F, Dobrossy M, Klein C. Front Cell Neurosci. 2016 Oct 24;10:245. eCollection 2016. PubMed
Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium. Du J, Yanagida A, Knight K, Engel AL, Vo AH, Jankowski C, Sadilek M, Tran VT, Manson MA, Ramakrishnan A, Hurley JB, Chao JR. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14710-14715. doi: 10.1073/pnas.1604572113. Epub 2016 Dec 1. PubMed
Interspecies Chimerism with Mammalian Pluripotent Stem Cells. Wu J, Platero-Luengo A, Sakurai M, Sugawara A, Gil MA, Yamauchi T, Suzuki K, Bogliotti YS, Cuello C, Morales Valencia M, Okumura D, Luo J, Vilarino M, Parrilla I, Soto DA, Martinez CA, Hishida T, Sanchez-Bautista S, Martinez-Martinez ML, Wang H, Nohalez A, Aizawa E, Martinez-Redondo P, Ocampo A, Reddy P, Roca J, Maga EA, Esteban CR, Berggren WT, Nunez Delicado E, Lajara J, Guillen I, Guillen P, Campistol JM, Martinez EA, Ross PJ, Izpisua Belmonte JC. Cell. 2017 Jan 26;168(3):473-486.e15. doi: 10.1016/j.cell.2016.12.036. PubMed
Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, Xu Y, Dong J, Wang C, Lai W, Zhu J, Xiong L, Zhu D, Li X, Yang W, Yamauchi T, Sugawara A, Li Z, Sun F, Li X, Li C, He A, Du Y, Wang T, Zhao C, Li H, Chi X, Zhang H, Liu Y, Li C, Duo S, Yin M, Shen H, Belmonte JCI, Deng H. Cell. 2017 Apr 6;169(2):243-257.e25. doi: 10.1016/j.cell.2017.02.005. PubMed
Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Cha Y, Han MJ, Cha HJ, Zoldan J, Burkart A, Jung JH, Jang Y, Kim CH, Jeong HC, Kim BG, Langer R, Kahn CR, Guarente L, Kim KS. Nat Cell Biol. 2017 May;19(5):445-456. doi: 10.1038/ncb3517. Epub 2017 Apr 24. PubMed
Transfection in perfused microfluidic cell culture devices: A case study. Raimes W, Rubi M, Super A, Marques MPC, Veraitch F, Szita N. Process Biochem. 2017 Aug;59(Pt B):297-302. doi: 10.1016/j.procbio.2016.09.006. PubMed
Patient-derived iPSCs show premature neural differentiation and neuron type-specific phenotypes relevant to neurodevelopment. Yeh E, Dao DQ, Wu ZY, Kandalam SM, Camacho FM, Tom C, Zhang W, Krencik R, Rauen KA, Ullian EM, Weiss LA. Mol Psychiatry. 2017 Nov 21. pii: mp2017238. doi: 10.1038/mp.2017.238. PubMed
Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming. Thekkeparambil Chandrabose S, Sriram S, Subramanian S, Cheng S, Ong WK, Rozen S, Kasim NHA, Sugii S. Stem Cell Res Ther. 2018 Mar 20;9(1):68. doi: 10.1186/s13287-018-0796-2. PubMed
The COPII cargo adapter SEC24C is essential for neuronal homeostasis. Wang B, Joo JH, Mount R, Teubner BJW, Krenzer A, Ward AL, Ichhaporia VP, Adams EJ, Khoriaty R, Peters ST, Pruett-Miller SM, Zakharenko SS, Ginsburg D, Kundu M. J Clin Invest. 2018 Aug 1;128(8):3319-3332. doi: 10.1172/JCI98194. Epub 2018 Jun 25. PubMed
SIRT2 is required for efficient reprogramming of mouse embryonic fibroblasts toward pluripotency. Kim AY, Lee EM, Lee EJ, Kim JH, Suk K, Lee E, Hur K, Hong YJ, Do JT, Park S, Jeong KS. Cell Death Dis. 2018 Aug 30;9(9):893. doi: 10.1038/s41419-018-0920-3. PubMed
The 7q11.23 Protein DNAJC30 Interacts with ATP Synthase and Links Mitochondria to Brain Development. Tebbenkamp ATN, Varela L, Choi J, Paredes MI, Giani AM, Song JE, Sestan-Pesa M, Franjic D, Sousa AMM, Liu ZW, Li M, Bichsel C, Koch M, Szigeti-Buck K, Liu F, Li Z, Kawasawa YI, Paspalas CD, Mineur YS, Prontera P, Merla G, Picciotto MR, Arnsten AFT, Horvath TL, Sestan N. Cell. 2018 Nov 1;175(4):1088-1104.e23. doi: 10.1016/j.cell.2018.09.014. PubMed
Integrated generation of induced pluripotent stem cells in a low-cost device. Lin H, Li Q, Du Q, Wang O, Wang Z, Akert L, Carlson MA, Zhang C, Subramanian A, Zhang C, Lunning M, Li M, Lei Y. Biomaterials. 2019 Jan;189:23-36. doi: 10.1016/j.biomaterials.2018.10.027. Epub 2018 Oct 25. PubMed
Cardiac Targeting Peptide, a Novel Cardiac Vector: Studies in Bio-Distribution, Imaging Application, and Mechanism of Transduction. Zahid M, Feldman KS, Garcia-Borrero G, Feinstein TN, Pogodzinski N, Xu X, Yurko R, Czachowski M, Wu YL, Mason NS, Lo CW. Biomolecules. 2018 Nov 14;8(4). pii: biom8040147. doi: 10.3390/biom8040147. PubMed
Generation of transgene-free porcine intermediate type induced pluripotent stem cells. Li D, Secher J, Hyttel P, Ivask M, Kolko M, Hall VJ, Freude KK. Cell Cycle. 2018;17(23):2547-2563. doi: 10.1080/15384101.2018.1548790. Epub 2018 Dec 3. PubMed
Mbd3 Promotes Reprogramming of Primary Human Fibroblasts. Jaffer S, Goh P, Abbasian M, Nathwani AC. Int J Stem Cells. 2018 Nov 30;11(2):235-241. doi: 10.15283/ijsc18036. PubMed
Distinct pathological signatures in human cellular models of myotonic dystrophy subtypes. Kim EY, Barefield DY, Vo AH, Gacita AM, Schuster EJ, Wyatt EJ, Davis JL, Dong B, Sun C, Page P, Dellefave-Castillo L, Demonbreun A, Zhang HF, McNally EM. JCI Insight. 2019 Mar 21;4(6). pii: 122686. doi: 10.1172/jci.insight.122686. eCollection 2019 Mar 21. PubMed
Embryonic Chimeras with Human Pluripotent Stem Cells. De Los Angeles A, Sakurai M, Wu J. Methods Mol Biol. 2019;2005:125-151. doi: 10.1007/978-1-4939-9524-0_9. PubMed
Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR. Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 10.1016/j.stem.2019.10.002. PubMed

Associated Plasmids

Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells. Kitajima R, Nakai R, Imamura T, Kameda T, Kozuka D, Hirai H, Ito H, Imai H, Imamura M. Stem Cell Res. 2020 Apr;44:101749. doi: 10.1016/j.scr.2020.101749. Epub 2020 Feb 28. PubMed
Establishment of an iPSC line (JTUi002-A) from a patient with Waardenburg syndrome caused by a SOX10 mutation and carrying a GJB2 mutation. Wang P, Wang J, Xing Y, Wang H, Yu D, Feng Y, Wu H, Wu Y, Chen Z, Wang J, Shi H. Stem Cell Res. 2020 Apr;44:101756. doi: 10.1016/j.scr.2020.101756. Epub 2020 Mar 7. PubMed
Establishment of an induced pluripotent stem cell line from a patient with CHARGE syndrome carrying a CHD7 (p.L1151Gfs*17) mutation. He S, Hu J, Zheng Z, Wang J, Chen J, Zhang C, Li L, Wang J, Chen Z, Shi H, Wang J. Stem Cell Res. 2020 May;45:101774. doi: 10.1016/j.scr.2020.101774. Epub 2020 Mar 20. PubMed
Deriving Keratinocyte Progenitor Cells and Keratinocytes from Human-Induced Pluripotent Stem Cells. Ibrahim MR, Medhat W, El-Fakahany H, Abdel-Raouf H, Snyder EY. Curr Protoc Stem Cell Biol. 2020 Sep;54(1):e119. doi: 10.1002/cpsc.119. PubMed
Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood Mononuclear Cells. Febbraro F, Chen M, Denham M. Methods Mol Biol. 2021;2239:135-151. doi: 10.1007/978-1-0716-1084-8_9. PubMed
Establishment of an iPSC line (CSUXHi003-A) from a patient with Waardenburg syndrome type caused by a MITF mutation. Wen J, Song J, He C, Ling J, Liu Y, Chen H, Gong W, Mei L, Feng Y. Stem Cell Res. 2021 Mar;51:102157. doi: 10.1016/j.scr.2021.102157. Epub 2021 Jan 9. PubMed
Monitoring Axonal Degeneration in Human Pluripotent Stem Cell Models of Hereditary Spastic Paraplegias. Li XJ, Mou Y, Milton C, Chen Z. Methods Mol Biol. 2021 Mar 27. doi: 10.1007/7651_2021_379. PubMed
Establishment of an iPSC line (CSUXHi004-A) from a patient with Waardenburg syndrome type I caused by a PAX3 splice mutation. Wen J, He C, Feng Y, Song J, Liu J, Liu X, Mei L, Ling J, Chen H, Liu Y. Stem Cell Res. 2021 May;53:102300. doi: 10.1016/j.scr.2021.102300. Epub 2021 Mar 18. PubMed
Generation of two induced pluripotent stem cell lines from patients with X-linked Alport syndrome. Ma Y, Wang Z, Gao M, Liu X, Sun W, Gong Y, Sun G, Liu G. Stem Cell Res. 2021 May;53:102343. doi: 10.1016/j.scr.2021.102343. Epub 2021 Apr 17. PubMed
Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog. Yoshimatsu S, Edamura K, Yoshii Y, Iguchi A, Kondo H, Shibuya H, Sato T, Shiozawa S, Okano H. Stem Cell Res. 2021 May;53:102375. doi: 10.1016/j.scr.2021.102375. Epub 2021 Apr 29. PubMed
Generation of human induced pluripotent stem cell line EURACi006-A and its isogenic gene-corrected line EURACi006-A-1 from an arrhythmogenic cardiomyopathy patient carrying the c.1643delG PKP2 mutation. Meraviglia V, Ganesh S, Arendzen CH, Freund C, Sommariva E, Rossini A, Bellin M. Stem Cell Res. 2021 Jul;54:102426. doi: 10.1016/j.scr.2021.102426. Epub 2021 Jun 8. PubMed
Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions. Perriot S, Canales M, Mathias A, Du Pasquier R. STAR Protoc. 2022 Aug 17;3(3):101620. doi: 10.1016/j.xpro.2022.101620. eCollection 2022 Sep 16. PubMed
Biofabrication of synthetic human liver tissue with advanced programmable functions. Florentino RM, Morita K, Haep N, Motomura T, Diaz-Aragon R, Faccioli LAP, Collin de l'Hortet A, Cetin Z, Frau C, Vernetti L, Amler AK, Thomas A, Lam T, Kloke L, Takeishi K, Taylor DL, Fox IJ, Soto-Gutierrez A. iScience. 2022 Nov 4;25(12):105503. doi: 10.1016/j.isci.2022.105503. eCollection 2022 Dec 22. PubMed
Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein. Green EA, Hamaker NK, Lee KH. BMC Biotechnol. 2023 Mar 7;23(1):7. doi: 10.1186/s12896-023-00777-7. PubMed
Targeted viral adaptation generates a simian-tropic hepatitis B virus that infects marmoset cells. Liu Y, Cafiero TR, Park D, Biswas A, Winer BY, Cho CH, Bram Y, Chandar V, Connell AKO, Gertje HP, Crossland N, Schwartz RE, Ploss A. Nat Commun. 2023 Jun 16;14(1):3582. doi: 10.1038/s41467-023-39148-3. PubMed

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