Addgene - eTC GFP beta-actin full length Plasmid Data

Plasmid 27123: eTC GFP beta-actin full length

Gene/insert name:
beta-actin
Species:
H. sapiens (human)
Entrez Gene:
ACTB (BRWS1, PS1TP5BP1)
Fusion protein or tag:
TC tag
Terminal:
N terminal on insert
Fusion protein or tag:
EGFP
Terminal:
N terminal on insert
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
HindIII
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Yes
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (4)
Map:
View map
Principal Investigator:
Robert Singer
Terms and Licenses:
MTA

Comments:

Note: Addgene's sequencing results indicate the presence of several nucleotide inserts (starting at bp# 2459, 2501 and 2516) and several gaps (at bp# 5352-5386) when compared to the sequence provided by the depositing laboratory. The depositing scientist confirmed that our sequencing result is correct and the inserts do not affect the function of the plasmid.

The plasmid map and sequence from the depositing laboratory lists EcoRI as the 3' cloning site; however, this site was destroyed during cloning. Please see the Addgene's sequencing results for cloning details.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Visualization of mRNA translation in living cells. Rodriguez et al (J Cell Biol. 2006 Oct 9. 175(1):67-76. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27123" in your Materials and Methods section.