Addgene - pJM614-TRA-2::GFP Plasmid Data

Plasmid 27128: pJM614-TRA-2::GFP

Gene/insert name:
TRA-2 GFP
Alt name:
C15F1.3
Insert size:
14645
Species:
C. elegans (nematode)
GenBank ID:
S42187
Entrez Gene:
tra-2 (C15F1.3)
Fusion protein or tag:
GFP
Terminal:
C terminal on insert
Mutation:
replaced stop codon with AgeI and EcoRI added GFP as same
Vector backbone:
pPD95.79
(Search Vector Database)
Vector type:
Worm Expression
Backbone size w/o insert (bp):
2700
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
SpeI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Ding Xue
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Somatic sex determination in Caenorhabditis elegans is modulated by SUP-26 repression of tra-2 translation. Mapes et al (Proc Natl Acad Sci U S A. 2010 Oct 19. 107(42):18022-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27128" in your Materials and Methods section.

Price: $65.00

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