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pJM601-TRA-2::FLAG
(Plasmid #27129)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27129 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD95.79
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRA-2 FLAG
  • Alt name
    C15F1.3
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    13801
  • Mutation
    Replaced TRA-2 stop codon with KpnI and EcoRI and inserted 3 copies of the FLAG tag
  • GenBank ID
    S42187
  • Entrez Gene
    tra-2 (a.k.a. CELE_C15F1.3)
  • Tag / Fusion Protein
    • 3x FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning of this plasmid was achieved in the following steps:

(1)the sequence 3' (3'UTR) of the tags was added by (EcoRI/SpeI)
(2) 1.2 kb XhoI/KpnI tra-2 pcr fragment into SalI/KpnI cut vector (destroying one SalI/XhoI site but adding another in the insert) immediately 5' of the tag
(3) 7 kb HindIII/SalI fragment into same that partially replaced fragment added in step 2 containing most of the TRA-2 coding sequence
(4) 5 kb Xho/AgeI that added the promoter and upstream gene in the operon as well as some Tra-2 coding sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJM601-TRA-2::FLAG was a gift from Ding Xue (Addgene plasmid # 27129 ; http://n2t.net/addgene:27129 ; RRID:Addgene_27129)
  • For your References section:

    Somatic sex determination in Caenorhabditis elegans is modulated by SUP-26 repression of tra-2 translation. Mapes J, Chen JT, Yu JS, Xue D. Proc Natl Acad Sci U S A. 2010 Oct 19. 107(42):18022-7. 10.1073/pnas.1004513107 PubMed 20921392