pCGN-ATF6 (1-373)m1
(Plasmid #27174)

• Purpose: For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.
• Depositing Lab: Ron Prywes
• Publication: Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-20.

Backbone

• Vector backbone: pCGN
• Backbone size w/o insert (bp): 7500
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATF6
• Species: H. sapiens (human)
• Insert Size (bp): 1119
• Mutation: Contains only aa 1-373 Amino acids 315–317 are changed from KNR to TAA
• Entrez Gene: ATF6 (a.k.a. ACHM7, ATF6A, ATP6alpha)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the dominant negative form of ATF6