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pcDNA-FLAG-Hu-Trip6
(Plasmid #27255)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27255 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trip6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1425
  • Entrez Gene
    TRIP6 (a.k.a. OIP-1, OIP1, TRIP-6, TRIP6i2, ZRP-1)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Trip6 cDNA contains starts at aa 2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-FLAG-Hu-Trip6 was a gift from Thomas Gilmore (Addgene plasmid # 27255 ; http://n2t.net/addgene:27255 ; RRID:Addgene_27255)
  • For your References section:

    LIM domain-containing protein trip6 can act as a coactivator for the v-Rel transcription factor. Zhao MK, Wang Y, Murphy K, Yi J, Beckerle MC, Gilmore TD. Gene Expr. 1999 . 8(4):207-17. PubMed 10794523