Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pSG-3'Hu-RelA
(Plasmid #27276)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27276 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSG424
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 3396
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RelA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    792
  • Mutation
    Contains only the transactivation domain (aa 292-551)
  • Entrez Gene
    RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
  • Tag / Fusion Protein
    • GAL4 (aa1-147) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Gal4 N-term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG-3'Hu-RelA was a gift from Thomas Gilmore (Addgene plasmid # 27276 ; http://n2t.net/addgene:27276 ; RRID:Addgene_27276)
  • For your References section:

    GAL4-I kappa B alpha and GAL4-I kappa B gamma activate transcription by different mechanisms. Morin PJ, Subramanian GS, Gilmore TD. Nucleic Acids Res. 1993 May 11. 21(9):2157-63. 10.1093/nar/21.9.2157 PubMed 8502557