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pcDNA3.1 AKT3 HA (CT-gfp)
(Plasmid #27293)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27293 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/CT-GFP-Topo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6157
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    protein kinase B gamma
  • Alt name
    akt3
  • Alt name
    Pkb gamma
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Mutation
    Y473F
  • Entrez Gene
    Akt3 (a.k.a. AI851531, D930002M15Rik, Nmf350)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstxI (not destroyed)
  • 3′ cloning site BstxI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Edge Biosystems
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GFP is not expressed as there is a stop codon after the HA tag.

mTORC2 reportedly can phosphorylate FSY and FSF sites, so the Y473F mutation should not affect expression or function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 AKT3 HA (CT-gfp) was a gift from Morris Birnbaum (Addgene plasmid # 27293 ; http://n2t.net/addgene:27293 ; RRID:Addgene_27293)