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pGL4.11 promPDE4D3 Ebox mut
(Plasmid #27304)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27304 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    prom PDE4D3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1730
  • Mutation
    Ebox CACATG changed to GAGATG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer RVI
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.11 promPDE4D3 Ebox mut was a gift from David Fisher (Addgene plasmid # 27304 ; http://n2t.net/addgene:27304 ; RRID:Addgene_27304)
  • For your References section:

    Control of melanocyte differentiation by a MITF-PDE4D3 homeostatic circuit. Khaled M, Levy C, Fisher DE. Genes Dev. 2010 Oct 15. 24(20):2276-81. 10.1101/gad.1937710 PubMed 20952536