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pcDNA3/mPax3-3xHA
(Plasmid #27319)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27319 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pax3
  • Alt name
    Pax-3
  • Alt name
    Paired Box Gene 3
  • Alt name
    Splotch
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1600
  • GenBank ID
    X59358
  • Entrez Gene
    Pax3 (a.k.a. Pax-3, Sp, Splchl2, splotch)
  • Tag / Fusion Protein
    • 3X HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Pax3 insert was derived from pBH3.2 Pax3 plasmid from Martyn Goulding, Max Plank Institute (Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P. Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. EMBO J. 1991 10(5):1135-47).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/mPax3-3xHA was a gift from Jonathan Epstein (Addgene plasmid # 27319 ; http://n2t.net/addgene:27319 ; RRID:Addgene_27319)
  • For your References section:

    Pax3 inhibits myogenic differentiation of cultured myoblast cells. Epstein JA, Lam P, Jepeal L, Maas RL, Shapiro DN. J Biol Chem. 1995 May 19. 270(20):11719-22. 10.1074/jbc.270.20.11719 PubMed 7744814