Addgene - pMRS001.2-GFP (control sensor) Plasmid Data

Plasmid 27405: pMRS001.2-GFP (control sensor)

Gene/insert name:
None
Fusion protein or tag:
GFP
Terminal:
C terminal on backbone
Vector backbone:
pCGTAG
(Search Vector Database)
5' sequencing primer:
M13 reverse List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
Mach1
Growth temperature (℃):
37
Growth instructions:
MACH-1 strain of E. coli (from Invitrogen)
High or low copy:
Unknown
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA

Comments:

The control sensor construct was created by cloning the potato UBI3 promoter into pENTR/D-TOPO (Invitrogen). The resulting plasmid was then recombined with pCGTAG. The promoter results in a ubiquitously expressed nuclear localized GFP.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: MicroRNAs prevent precocious gene expression and enable pattern formation during plant embryogenesis. Nodine et al (Genes Dev. 2010 Dec 1. 24(23):2678-92. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 27405" in your Materials and Methods section.