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CtkA_D155Q/D179Q_pET21a
(Plasmid #27432)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 27432 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cell translocating kinas A
  • Alt name
    CtkA
  • Alt name
    Jhp0940
  • Species
    H. pylori
  • Insert Size (bp)
    975
  • Mutation
    D155Q/D179Q
  • Entrez Gene
    jhp0940
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CtkA_D155Q/D179Q_pET21a was a gift from SeWon Suh (Addgene plasmid # 27432 ; http://n2t.net/addgene:27432 ; RRID:Addgene_27432)
  • For your References section:

    Helicobacter pylori proinflammatory protein up-regulates NF-{kappa}B as a cell-translocating Ser/Thr kinase. Kim DJ, Park KS, Kim JH, Yang SH, Yoon JY, Han BG, Kim HS, Lee SJ, Jang JY, Kim KH, Kim MJ, Song JS, Kim HJ, Park CM, Lee SK, Lee BI, Suh SW. Proc Natl Acad Sci U S A. 2010 Nov 22. ():. 10.1073/pnas.1010153107 PubMed 21098302