pETZZtevPP7CP(C68A,C73A)
(Plasmid #27548)

• Depositing Lab: Kathleen Collins
• Publication: Hogg et al RNA. 2007 Jun . 13(6):868-80.

Backbone

• Vector backbone: pET28
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pseudomonas phage PP7 coat protein
• Alt name: PP7CP
• Species: Pseudomonas phage PP7
• Insert Size (bp): 900
• Mutation: changed two cysteine to alanine (C68A,C73A) to inhibit coat protein polymerization into capsid
• GenBank ID: CAA56473
• Entrez Gene: cp (a.k.a. PP7p2)
• Tags / Fusion Proteins:
  • tandem protein A (ZZ) (N terminal on insert)
  • TEV protease cleavage site (N terminal on insert)
  • six histidine tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 Fwd

Resource Information

• Supplemental Documents:
  • Coat Protein Purification Protocol
• A portion of this plasmid was derived from a plasmid made by: David Peabody
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry