pCLXSN-Tpl2/Cot
(Plasmid #27558)

• Depositing Lab: Shao-Cong Sun
• Publication: Babu et al J Biol Chem. 2006 May 19. 281(20):14041-7.

Backbone

• Vector backbone: pCLXSN
• Backbone manufacturer: Addgene Plasmid 12343 (Dr. Inder Verma)
• Backbone size w/o insert (bp): 7807
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tpl2/Cot
• Alt name: Tpl2
• Species: H. sapiens (human)
• Entrez Gene: MAP3K8 (a.k.a. AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PCLXSN-based plasmids were constructed using the pCLXSN vector obtained from Dr. Inder Verma (do not remember if there is MTA)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry