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pLenti CMVTRE3G Neo DEST (w813-1)
(Plasmid #27566)

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size (bp) 9621
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1, 37oC
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Empty

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The CMVTRE3G promoter was a gift from Dr. Dominic Esposito (SAIC Frederick). Tet-On 3G compatible plasmid.
  • Terms and Licenses

Depositor Comments

There are a few mismatches between Addgene's quality control sequence and depositor's reference sequence. However the changes are in vector sequence only, features unaffected. Depositor tested this construct and it was functional.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMVTRE3G Neo DEST (w813-1) was a gift from Eric Campeau (Addgene plasmid # 27566)