pLenti CMVTRE3G Neo DEST (w813-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 9621
Vector typeMammalian Expression, Lentiviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsDB3.1, 37oC
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVtight
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe CMVTRE3G promoter was a gift from Dr. Dominic Esposito (SAIC Frederick). Tet-On 3G compatible plasmid.
Terms and Licenses
There are a few mismatches between Addgene's quality control sequence and depositor's reference sequence. However the changes are in vector sequence only, features unaffected. Depositor tested this construct and it was functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMVTRE3G Neo DEST (w813-1) was a gift from Eric Campeau (Addgene plasmid # 27566)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.