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pQCXIN- myc ULK1 4SA
(Plasmid #27628)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27628 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQCXIN Neo dest
  • Backbone manufacturer
    Addgene plasmid 17399
  • Backbone size w/o insert (bp) 9135
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse myc ULK1 4SA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3192
  • Mutation
    S467A, S555A, T574A, S637A. Initial M from ULK1 removed. Silent mutation at Arginine 5, which does not affect amino acid sequence.
  • Entrez Gene
    Myc (a.k.a. AU016757, Myc2, Niard, Nird, bHLHe39)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site none/attR1 (unknown if destroyed)
  • 3′ cloning site none/attR2 (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIN- myc ULK1 4SA was a gift from Reuben Shaw (Addgene plasmid # 27628 ; http://n2t.net/addgene:27628 ; RRID:Addgene_27628)
  • For your References section:

    Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Science. 2010 Dec 23. ():. 10.1126/science.1196371 PubMed 21205641