pcdna6.2- myc ULK1 k46I
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27630||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA 6.2 V5 dest (Invitrogen)
- Backbone size w/o insert (bp) 7341
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert namemouse myc ULK1 K46I
SpeciesM. musculus (mouse)
Insert Size (bp)3192
MutationInitial M from ULK1 removed. Silent mutation at Arginine 5. Does not affect amino acid sequence.K46I mutation makes kinase catalytically inactive.
Entrez GeneMyc (a.k.a. AU016757, Myc2, Niard, Nird, bHLHe39)
/ Fusion Protein
- myc (N terminal on insert)
- Cloning method Gateway Cloning
- 5′ cloning site none/attR1 (unknown if destroyed)
- 3′ cloning site none/attR2 (unknown if destroyed)
- 5′ sequencing primer t7 promotor (Common Sequencing Primers)
C-term V5 tag not expressed because stop codon at end of ULK1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcdna6.2- myc ULK1 k46I was a gift from Reuben Shaw (Addgene plasmid # 27630 ; http://n2t.net/addgene:27630 ; RRID:Addgene_27630)
For your References section:Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Science. 2010 Dec 23. ():. 10.1126/science.1196371 PubMed 21205641