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(Plasmid #27651)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 27651 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    M. Gossen et al., 1992 (PNAS)
  • Backbone size w/o insert (bp) 3150
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    D146N (Dominant Negative)
  • Entrez Gene
    CDK2 (a.k.a. CDKN2, p33(CDK2))
  • Tag / Fusion Protein
    • HA (YDVPDYA) (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See attachment for description of backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUHD Cdk2 DN HA was a gift from Greg Enders (Addgene plasmid # 27651 ; ; RRID:Addgene_27651)
  • For your References section:

    S and G2 phase roles for Cdk2 revealed by inducible expression of a dominant-negative mutant in human cells. Hu B, Mitra J, van den Heuvel S, Enders GH. Mol Cell Biol. 2001 Apr . 21(8):2755-66. 10.1128/MCB.21.8.2755-2766.2001 PubMed 11283255