pUHD Cdk2 DN HA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27651||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerM. Gossen et al., 1992 (PNAS)
- Backbone size w/o insert (bp) 3150
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationD146N (Dominant Negative)
Entrez GeneCDK2 (a.k.a. CDKN2, p33(CDK2))
/ Fusion Protein
- HA (YDVPDYA) (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX
- 3′ sequencing primer EBV_Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See attachment for description of backbone.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUHD Cdk2 DN HA was a gift from Greg Enders (Addgene plasmid # 27651 ; http://n2t.net/addgene:27651 ; RRID:Addgene_27651)
For your References section:S and G2 phase roles for Cdk2 revealed by inducible expression of a dominant-negative mutant in human cells. Hu B, Mitra J, van den Heuvel S, Enders GH. Mol Cell Biol. 2001 Apr . 21(8):2755-66. 10.1128/MCB.21.8.2755-2766.2001 PubMed 11283255