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pBABEpuro U3-hTR-500
(Plasmid #27666)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27666 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABEpuro
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U3-hTR-500
  • Alt name
    human telomerase RNA
  • Alt name
    TER
  • Alt name
    TERC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    TERC (a.k.a. DKCA1, PFBMFT2, SCARNA19, TER, TR, TRC3, hTR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sequence is for the insert of human U3 snoRNA promoter driving expression of human telomerase RNA mature sequence (hTR) with 500 bp downstream genomic region included; see Fu and Collins (2003)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEpuro U3-hTR-500 was a gift from Kathleen Collins (Addgene plasmid # 27666 ; http://n2t.net/addgene:27666 ; RRID:Addgene_27666)
  • For your References section:

    Telomerase RNA level limits telomere maintenance in X-linked dyskeratosis congenita. Wong JM, Collins K. Genes Dev. 2006 Oct 15. 20(20):2848-58. 10.1101/gad.1476206 PubMed 17015423