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hWIP1 FLAG
(Plasmid #28105)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28105 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone size w/o insert (bp) 7767
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WIP1
  • Alt name
    wild type p53 induced phosphatase
  • Alt name
    PPM1D
  • Alt name
    protein phosphatase magnesium-dependent 1 delta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1865
  • Entrez Gene
    PPM1D (a.k.a. IDDGIP, JDVS, PP2C-DELTA, WIP1)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hWIP1 FLAG was a gift from Lawrence Donehower (Addgene plasmid # 28105 ; http://n2t.net/addgene:28105 ; RRID:Addgene_28105)
  • For your References section:

    Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. Moon SH, Lin L, Zhang X, Nguyen TA, Darlington Y, Waldman AS, Lu X, Donehower LA. J Biol Chem. 2010 Apr 23. 285(17):12935-47. 10.1074/jbc.M109.071696 PubMed 20118229