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PHF13 (3O7A)
(Plasmid #28155)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28155 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28-MHL (Addgene plasmid 26096)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHD finger protein 13
  • Alt name
    PDB: 3O7A
  • Alt name
    PHD finger protein 13, PHD-type zinc finger domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    174
  • Mutation
    PHD-type zinc finger domain (residues 250-301.
  • GenBank ID
    BC038516
  • Entrez Gene
    PHF13 (a.k.a. PHF5, SPOC1)
  • Tag / Fusion Protein
    • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PHF13 (3O7A) was a gift from Cheryl Arrowsmith (Addgene plasmid # 28155 ; http://n2t.net/addgene:28155 ; RRID:Addgene_28155)