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pBS U3-hTR-500
(Plasmid #28170)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28170 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U3-hTR-500
  • Alt name
    human telomerase RNA
  • Alt name
    TER
  • Alt name
    TERC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    TERC (a.k.a. DKCA1, PFBMFT2, SCARNA19, TER, TR, TRC3, hTR)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that BLAST results bring back one T->C nt change in the TERC, but this is the plasmid used in the associated article.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS U3-hTR-500 was a gift from Kathleen Collins (Addgene plasmid # 28170 ; http://n2t.net/addgene:28170 ; RRID:Addgene_28170)
  • For your References section:

    Distinct biogenesis pathways for human telomerase RNA and H/ACA small nucleolar RNAs. Fu D, Collins K. Mol Cell. 2003 May . 11(5):1361-72. 10.1016/S1097-2765(03)00196-5 PubMed 12769858