|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28175||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepGL3-control vector
- Backbone size w/o insert (bp) 5256
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLuciferase flanked by tp53 UTRs
SpeciesH. sapiens (human)
Insert Size (bp)1352
MutationNote that insert length is based on sum of the 2 UTRs (145+1207)
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII/NcoI (5'UTR) (not destroyed)
- 3′ cloning site XbaI (3'UTR) (not destroyed)
- 5′ sequencing primer pGL3ctrl luc spb1
- 3′ sequencing primer pGL3 spb2 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:145-pGL3ctrl-3' UTR was a gift from Michael Kastan (Addgene plasmid # 28175 ; http://n2t.net/addgene:28175 ; RRID:Addgene_28175)
For your References section:5'-3'-UTR interactions regulate p53 mRNA translation and provide a target for modulating p53 induction after DNA damage. Chen J, Kastan MB. Genes Dev. 2010 Oct 1. 24(19):2146-56. 10.1101/gad.1968910 PubMed 20837656
Map uploaded by Addgene staff.