145-pGL3ctrl-3' UTR
(Plasmid #28175)

• Depositing Lab: Michael Kastan
• Publication: Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

Backbone

• Vector backbone: pGL3-control vector
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5256
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Luciferase flanked by tp53 UTRs
• Species: H. sapiens (human)
• Insert Size (bp): 1352
• Mutation: Note that insert length is based on sum of the 2 UTRs (145+1207)
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII/NcoI (5'UTR) (not destroyed)
• 3′ cloning site: XbaI (3'UTR) (not destroyed)
• 5′ sequencing primer: pGL3ctrl luc spb1
• 3′ sequencing primer: pGL3 spb2

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry