pCMV5M/rDlg1
(Plasmid #28259)

• Depositing Lab: Jeffrey Frost
• Publication: Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-80.

Backbone

• Vector backbone: pCMV5M
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dlg1 I3
• Alt name: disks large homolog 1, I3 splice variant
• Species: R. norvegicus (rat)
• Insert Size (bp): 2700
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: this Dlg1 splice variant differs from Dlg1 GenBank entry NP_036920.1 (Swiss-Prot Q62969) by a deletion of 33 amino acids corresponding to aa# 162-194 in the GenBank entry.