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pcDNA3.1-ERBB4 (E452K)
(Plasmid #29529)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    ERBB4
  • Alt name
    HER4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3927
  • Mutation
    changed glutamic acid 452 to lysine
  • GenBank ID
    NM_005235
  • Entrez Gene
    ERBB4 (a.k.a. ALS19, HER4, p180erbB4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-ERBB4 (E452K) was a gift from Yardena Samuels (Addgene plasmid # 29529)
  • For your References section:

    Analysis of the tyrosine kinome in melanoma reveals recurrent mutations in ERBB4. Prickett TD, Agrawal NS, Wei X, Yates KE, Lin JC, Wunderlich JR, Cronin JC, Cruz P, Rosenberg SA, Samuels Y. Nat Genet. 2009 Oct . 41(10):1127-32. 10.1038/ng.438 PubMed 19718025