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pCDF1-ADAMTS18 (Q904X)
(Plasmid #29556)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCDF1-EF1-MCS2-Puromycin
  • Backbone manufacturer
    Systems Biosciences Inc
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    ADAMTS18
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3640
  • Mutation
    changed glutamine 904 to stop codon
  • GenBank ID
    NM_199355 BC063283
  • Entrez Gene
    ADAMTS18 (a.k.a. ADAMTS21, KNO2)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF1-ADAMTS18 (Q904X) was a gift from Yardena Samuels (Addgene plasmid # 29556)
  • For your References section:

    Mutational and functional analysis reveals ADAMTS18 metalloproteinase as a novel driver in melanoma. Wei X, Prickett TD, Viloria CG, Molinolo A, Lin JC, Cardenas-Navia I, Cruz P, Rosenberg SA, Davies MA, Gershenwald JE, Lopez-Otin C, Samuels Y. Mol Cancer Res. 2010 Nov . 8(11):1513-25. 10.1158/1541-7786.MCR-10-0262 PubMed 21047771