Gene/insert name: ADAMTS18
Insert size: 3640
Species:
H. sapiens (human)
GenBank ID:
NM_199355
BC063283
Entrez Gene:
ADAMTS18
(ADAMTS21, KNO2)
Fusion protein or tag: FLAG
Terminal: C terminal on insert
Mutation: changed glutamine 904 to stop codon
Vector backbone:
pCDF1-EF1-MCS2-PuromycinSearch Vector Database )
Backbone manufacturer:
Systems Biosciences Inc
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
5500
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
N/A
List of Sequencing Primers
3' sequencing primer:
N/A
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
Puromycin
Sequence:
View sequences (2)
Principal Investigator:
Yardena Samuels
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
29556" in your Materials and Methods section.
