Addgene - pDONRHYG Plasmid Data

Plasmid 29636: pDONRHYG

Gene/insert name:
None
Alt name:
DelsGate
Vector backbone:
pDONR201
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Gateway donor
Backbone size (bp):
4470
Cloning method:
Gateway Cloning
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
Unknown
Growth temperature (℃):
37
Growth instructions:
DB3.1 (ccdB resistant)
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
David_L Andrews
Principal Investigator:
Scott Gold
Terms and Licenses:
MTA

Comments:

This plasmid is a DelsGate vector that contains a selectable marker for the transformation of Basidiomycete fungi.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: DelsGate, a robust and rapid gene deletion construction method. Garcia-Pedrajas et al (Fungal Genet Biol. 2008 Apr . 45(4):379-88. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29636" in your Materials and Methods section.