Addgene - pENTR4-HaloTag (w876-1) Plasmid Data

Plasmid 29644: pENTR4-HaloTag (w876-1)

Gene/insert name:
no insert
Fusion protein or tag:
HaloTag
Terminal:
N terminal on backbone
Vector backbone:
pENTR4
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Entry vector
Backbone size (bp):
3221
Cloning site 5':
Nco I
Site destroyed during cloning:
No
Cloning site 3':
Bam HI
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

Sequence verified, no further characterization.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29644" in your Materials and Methods section.