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BirA in pENTR1A (w860-1)
(Plasmid #29645)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29645 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2266
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA
  • Alt name
    biotin ligase
  • Insert Size (bp)
    980

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (not destroyed)
  • 3′ cloning site Eco RV (not destroyed)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The BirA gene was amplified from the E.coli TOP10F' strain, sequence confirmed, and functionally tested in E.coli using a protein fused to an AviTag.

Please note that using this plasmid in a LR reaction with a Destination vector encoding an N-terminal tag is not recommended as it would not be in frame with the BirA sequence. This plasmid was designed to be used to biotinylate substrates in vivo. To add a tag to BirA, clone the desired tag into the vector itself, rather than using Gateway cloning--this approach will also prevent unnecessary sequence in the expressed protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BirA in pENTR1A (w860-1) was a gift from Eric Campeau (Addgene plasmid # 29645 ; http://n2t.net/addgene:29645 ; RRID:Addgene_29645)