Addgene - BirA in pENTR1A (w860-1) Plasmid Data

Plasmid 29645: BirA in pENTR1A (w860-1)

Gene/insert name:
BirA
Alt name:
biotin ligase
Insert size:
980
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Entry vector
Backbone size w/o insert (bp):
2266
Cloning site 5':
Eco RI
Site destroyed during cloning:
No
Cloning site 3':
Eco RV
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

The BirA gene was amplified from the E.coli TOP10F' strain, sequence confirmed, and functionally tested in E.coli using a protein fused to an AviTag.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29645" in your Materials and Methods section.