Addgene - pTH645-RLuc Plasmid Data

Plasmid 29694: pTH645-RLuc

Gene/insert name:
Renilla Luciferase
Alt name:
RLuc
Insert size:
948
Species:
Renilla muelleri
GenBank ID:
AF043450
Vector backbone:
pTH644_CENBEVY
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
6598
Cloning site 5':
XmaC1
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tobias von der Haar
Terms and Licenses:
MTA

Comments:

Please note that there are minor mismatches between depositor's reference sequence and Addgene's quality control sequence. All differences are outside of the cloned gene, and do not affect the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae. Chu et al (Nucleic Acids Res. 2011 May 9. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29694" in your Materials and Methods section.