Addgene - pTH651-RLuc/minFLuc Plasmid Data

Plasmid 29697: pTH651-RLuc/minFLuc

Gene/insert name:
Firefly Luciferase
Alt name:
FLuc
Insert size:
1659
Species:
Photinus pyralis
GenBank ID:
AF043450
Mutation:
All codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast
Vector backbone:
pTH645-RLuc
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
7534
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Tobias von der Haar
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae. Chu et al (Nucleic Acids Res. 2011 May 9. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 29697" in your Materials and Methods section.