pET28b-LipB
(Plasmid
#30165)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28b
- Backbone size w/o insert (bp) 5500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namelipoate protein ligase B
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Alt namelipB
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SpeciesThermus thermophilus HB27
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Insert Size (bp)651
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7-term
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b-LipB was a gift from SeWon Suh (Addgene plasmid # 30165 ; http://n2t.net/addgene:30165 ; RRID:Addgene_30165) -
For your References section:
Structural basis of octanoic acid recognition by lipoate-protein ligase B. Kim do J, Lee SJ, Kim HS, Kim KH, Lee HH, Yoon HJ, Suh SW. Proteins. 2008 Mar . 70(4):1620-5. 10.1002/prot.21843 PubMed 18076036